Preprint / Version 1

Detection of Class 1 Integron Among Klebsiella Species Isolated from Clinical Samples at No (1) Defence Services General Hospital (1000 Bedded)

Authors

  • Kyaw Khine Win Microbiology Department, Defence Services Medical Academy, Yangon, Myanmar
  • Khine Khine Su Microbiology Department, Defence Services Medical Academy, Yangon, Myanmar
  • Kaung Myat Thu No (2) Military Hospital (300- Bedded)
  • Kyaw Wunna Microbiology Department, Defence Services Medical Academy, Yangon, Myanmar
  • Nay Myo Aung Microbiology Department, Defence Services Medical Academy, Yangon, Myanmar
  • Aung Phyo Oo Microbiology Department, Defence Services Medical Academy, Yangon, Myanmar
  • Win Ko Ko Min Microbiology Department, Defence Services Medical Academy, Yangon, Myanmar
  • Aye Min Thant Microbiology Department, No. (1) Defence Services General Hospital, Yangon, Myanmar

DOI:

https://doi.org/10.21467/preprints.414

Abstract

Klebsiella species is commonly associated with serious nosocomial infections. Multi-drug resistant Klebsiella species isolates are becoming increasingly prevalent in the clinical and nosocomial environments. The high prevalence of Klebsiella infections is related to the ability of Klebsiella species to acquire and disseminate exogenous genes associated with mobile elements, such as plasmids, transposons and integrons. This study was conducted to find out the presence of class 1 integron and antibiotic susceptibility patterns of Klebsiella species from clinical specimens at No (1) Defence Services General Hospital (1000-Bedded). A laboratory based cross-sectional descriptive study was carried out from January to september, 2020. Identification and antimicrobial susceptibility testing of Klebsiella species was performed by VITEK 2 Compact Analyzer. Class 1 integrons were detected by conventional PCR. In this study, 110 Klebsiella species were isolated from various clinical specimens and most of the isolates were from medical ward (52.3%, 63 isolates). The highest rate of resistance was observed for ampicillin (100%) and Cefotaxime (97.3 %) whereas the lowest antibiotic resistance was to Amikacin (15.5%). Out of 110 Klebsiella isolates, 107 (97.3%) were multidrug resistant (MDR). Forty-four (40%) out of 110 Klebsiella isolates carried int1 gene and all these isolates were MDR. However, there was no association between multidrug resistance and integron positivity (p value = 0.273). The presence of class I integron genes among Klebsiella species highlights the continued monitoring is necessary for prevention of wide dissemination of integrons and infections by MDR pathogens.

Keywords:

Klebsiella, MDR, Class (1) integron, No. (1) DSGH (1000-Bedded)

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References

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Posted

2022-10-15

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