Preprint / Version 1

Detection of Class 1 Integron Among Klebsiella Species Isolated from Clinical Samples at No (1) Defence Services General Hospital (1000 Bedded)

Authors

  • Kaung Myat Thu No (2) Military Hospital (300- Bedded)
  • Kyaw Khine Win Microbiology Department, Defence Services Medical Academy, Yangon, Myanmar
  • Khine Khine Su Microbiology Department, Defence Services Medical Academy, Yangon, Myanmar
  • Kyaw Wunna Microbiology Department, Defence Services Medical Academy, Yangon, Myanmar
  • Nay Myo Aung Microbiology Department, Defence Services Medical Academy, Yangon, Myanmar
  • Aung Phyo Oo Microbiology Department, Defence Services Medical Academy, Yangon, Myanmar
  • Win Ko Ko Min Microbiology Department, Defence Services Medical Academy, Yangon, Myanmar
  • Aye Min Thant Microbiology Department, No. (1) Defence Services General Hospital, Yangon, Myanmar

DOI:

https://doi.org/10.21467/preprints.414

Abstract

Klebsiella species is commonly associated with serious nosocomial infections. Multi-drug resistant Klebsiella species isolates are becoming increasingly prevalent in the clinical and nosocomial environments. The high prevalence of Klebsiella infections is related to the ability of Klebsiella species to acquire and disseminate exogenous genes associated with mobile elements, such as plasmids, transposons and integrons. This study was conducted to find out the presence of class 1 integron and antibiotic susceptibility patterns of Klebsiella species from clinical specimens at No (1) Defence Services General Hospital (1000-Bedded). A laboratory based cross-sectional descriptive study was carried out from January to september, 2020. Identification and antimicrobial susceptibility testing of Klebsiella species was performed by VITEK 2 Compact Analyzer. Class 1 integrons were detected by conventional PCR. In this study, 110 Klebsiella species were isolated from various clinical specimens and most of the isolates were from medical ward (52.3%, 63 isolates). The highest rate of resistance was observed for ampicillin (100%) and Cefotaxime (97.3 %) whereas the lowest antibiotic resistance was to Amikacin (15.5%). Out of 110 Klebsiella isolates, 107 (97.3%) were multidrug resistant (MDR). Forty-four (40%) out of 110 Klebsiella isolates carried int1 gene and all these isolates were MDR. However, there was no association between multidrug resistance and integron positivity (p value = 0.273). The presence of class I integron genes among Klebsiella species highlights the continued monitoring is necessary for prevention of wide dissemination of integrons and infections by MDR pathogens.

Keywords:

Klebsiella, MDR, Class (1) integron, No. (1) DSGH (1000-Bedded)

Downloads

Download data is not yet available.

References

?1? K C Caroll, S A Morse, T Mietzner and S Miller. Klebsiella in Jawetz, Melnick & Adelberg’s (eds), Medical Microbiology, 27 th Edition, MC Graw Hill Education, New York, pp. 236 (2016)

?2? Gill M. K, Kaur A, and Khanna A (2019). Antibiogram of Klebsiella pneumoniae isolated from various clinical samples of hospitalized patients in a tertiary care hospital of North India. Tropical Journal of Pathology & Microbiology. 5 (8) 512-516.

?3? É. L. Fonseca and A. C. Vicente, “Integron Functionality and Genome Innovation: An Update on the Subtle and Smart Strategy of Integrase and Gene Cassette Expression Regulation,” Microorganisms, vol. 10, no. 2, p. 224, Jan. 2022, doi: 10.3390/microorganisms10020224. [Online]. Available: http://dx.doi.org/10.3390/microorganisms10020224

?4? S B Kor, Q C Choo, C H Chew. New integron gene arrays from multiresistant clinical isolates of members of the Enterobacteriaceae and Pseudomonas aeruginosa from hospitals in Malaysia. J Med Microbiol. 2013 Mar;62(Pt 3):412-420. doi: 10.1099/jmm.0.053645-0. Epub 2012 Nov 22. PMID: 23180481.

?5? S Mirkalantari, N Momeni, R Mirnejad and F Bineshian (2017). Assessment of the Prevalence of Class I and II Integrons in Klebsiella pneumoniae Isolates from Patients Referred to the Hospitals of Semnan, Iran. Journal of Applied Biotechnology Reports. 4 (4): 719-722.

?6? F Firoozeh , Z Mahluji , A Khorshidi and M Zibaei . (2019). Molecular characterization of class 1, 2 and 3 integrons in clinical multi-drug resistant Klebsiella pneumoniae isolates Antimicrobial Resistance and Infection Control. 8: 59.

?7? SJ Sonia, S Afroz, Md Rasheduzzaman, K H Uddin, and S M Shamsuzzaman (2020). Prevalence and Antimicrobial Susceptibility Pattern of Klebsiella Pneumoniae Isolated from Various Clinical Specimens in a Tertiary Care Hospital in Bangladesh. Medicine Today 32(02) 95- 99 DOI: https://doi.org/10.3329/medtoday.v32i2.48821

?8? S Ghanem, H M E Shafey, E K A Tamer and N Manzoor (2017). Antimicrobial resistance patterns of Klebsiella isolates from clinical samples in a Saudi hospital. African Journal of Microbiology Research. 11(23), 965-971.

?9? S G Tekele, D S Teklu, K D Tullu, S K Birru, M H Legese (2020) Extended-spectrum Beta-lactamase and AmpC beta-lactamases producing gram negative bacilli isolated from clinical specimens at International Clinical Laboratories, Addis Ababa, Ethiopia.

?10? H Virawan, T Nuryastuti, and H Nirwati (2020) Multidrug resistant Klebsiella pneumoniae from clinical isolates at dr. Soeradji Tirtonegoro central hospital Klaten Indonesian Journal of Medicine and Health. 11(2): 109-120

?11? P Shakib. (2020). Prevalence of Class 1 Integron in Klebsiella Pneumoniae Isolates from Hospitals of Sanandaj, Iran. International Journal of Medical Laboratory.

?12? Malekjamshidi M R, Zandi H and Eftekhar F (2018) Prevalence of Extended-Spectrum ?-lactamase and Integron Gene Carriage in Multidrug-Resistant Klebsiella Species Isolated from Outpatients in Yazd, Iran. Iran J Med Sci

?13? M Mobarak-Qamsari, M Ashayeri-Panah, F Eftekhar, and M M Feizabadi (2013). Integron mediated multidrug resistance in extended spectrum beta-lactamase producing clinical isolates of Klebsiella pneumoniae. Brazilian Journal of Microbiology 44, 3, 849-854

Downloads

Posted

2022-10-15

Section

Preprints

Categories