Viability of Lactic Acid Bacteria (L. Acidophilus) in Probiotic Ready to Drink Juices
DOI:
https://doi.org/10.21467/preprints.519Abstract
Probiotics are consumed in dairy based goods, however, given the rise in various diet types, non-dairy alternatives, such as inoculating fruit juices with probiotics were studied for substitute. Lactobacillus acidophilus is a probiotic strain exerting a number of human health benefits. Therefore, the objective of this study was to determine the viability of Lactobacillus acidophilus in different non-dairy substrate to produce a Probiotic Ready to drink (RTD) juice blend made of malunggay, pineapple and mango over 24 hours of fermentation and 21 days of refrigerated storage. The secondary objective was to determine the sensory parameters using 9-point Hedonic scale and physicochemical properties of probiotic RTD Juice Blend in terms of pH, TSS and total acidity. All of the probiotic RTD Juice Blend samples achieved a mean viable count of at least 106 CFU/mL during 24 hours of fermentation and 21 days of refrigerated storage. According to the sensory evaluation, which evaluated samples according to color, aroma, taste and overall acceptability, probiotic RTD juice blend with malunggay-pineapple-mango blend proved to have the highest score for all characteristics with pH of 4.62, TSS of 13.47OBx and total acidity of 0.47%. The pH and TSS decreases with time while acidity increases. This condition is good for the production of microbes because low pH can decrease the microbes’ production rate. This environment may enable LAB to successfully pass the pH of the stomach and bind in the gut of the host which is needed for successful colonization and propagation for expression of its health-promoting effects. Therefore, this study indicated a potential for probiotic fruit juices as a valid alternative to dairy based probiotic products.
Keywords:
Lactobacillus acidophilus, fruit and vegetables, ready-to-drinkDownloads
References
Sharma V. and Mishra, H. N. (2013). Fermentation of vegetable juice mixture by probiotic lactic acid bacteria. Nutra Foods, 12:17:22.
Salminen S, G. C. (2003). Gastrointestinal physiology and function: the role of prebiotics and probiotics. Br J Nutr.
Afifi, M. (2011). Enhancement of Lactic Acid Production by Utilizing Liquid Potato Wastes. International Journal of Biological Chemistry.
Mamaril, V. (2019.) Bawal ang magutom: Malunggay at your doorstep. BIOLIFE Magazine, 2009 (1):34-35.
Rathnayaka, Rmus (2018)“Antibacterial effect of malic acid against Listeria monocytogenes, Salmonella enteritidis and Escherichia coli in Mango, Pineapple and Papaya juices”, American Journal of Food Technology.; 8(1):74-82.
Shukla, M., Jha, Y. K., Admassu, S. (2021), Development of Probiotic Beverage from Whey and Pineapple Juice. Journal of Food Processing and Technology, Vol. 4, pp. 206.
Kleerebezem , M. , and Hugenholtz , J. ( 2022 ) Metabolic pathway engineering in lactic acid bacteria . Curr Opin Biotechnol 14, 232 – 237.
Barcelon, E., Isuga A., Macadangdang, M., Resurreccion I., Salipsip A., Tan, R., Torres, V. (2015). Proximate Content and Sensory Acceptability Of Carrot and SweetPotato – Filled Chocolate Bites Incorporated With L. Casei and L. plantarum. University of Santo Tomas, College of Education, Department of Food Technology, España Blvd., Sampaloc, 1008, Manila, Philippines
FAO. CODEX ALIMENTARIUS, (2016) “Joint FAOWHO food standards programmecodex committee on processed fruit and vegetables” [www.codexalimentarius.org/.../standards/../pfv28_crd2E.pdf]
Spicher G, Stephen HS. Hanbuch Sauerteig: Biologie, biochemie technologies, B. Behrs Verlag, Hamburg; 2020
Maskan M. Production of pomegranate (Punica granatum L.) juice concentrate by various heating methods: Colour degradation and kinetics. J. Fd. Eng. 2016;72:218-224.
Larmond E. Laboratory methods for sensory evaluation of food. Canada Department of Agriculture, Ottawa; 2017.
Mengesha, Y., Tebeje, A., & Tilahun, B. (2022). A Review on Factors Influencing the Fermentation Process of Teff (Eragrostis teff) and Other Cereal-Based Ethiopian Injera. International journal of food science, 4419955. https://doi.org/10.1155/2022/4419955
Viander, B., Maki, M., & Palva, A. (2020). Impact of low salt concentration, salt, quality on natural large-scale sauerkraut fermentation. Food Microbiology, 20, 391-395.
Kumar, B. V., Murthy M. P., Reddy, O. V. S. (2019). Physico-Chemical Analysis of Fresh and Fermented Fruit Juices Probioticated with Lactobacillus casei, International Journal of Applied Sciences and Biotechnology, Vol. 1(3), pp. 127-131.
Yoon, K. Y., Woodams, E. E., Hang, Y. D. (2015), Fermentation of beet juice by beneficial lactic acid bacteria. Food Science and Technology, Vol. 38(1), pp. 73-75.
[ 17] Mongkontanawat, N., Laohkitikul , S., & Lertnimitmongkol, W. (2018). Fermentation of Gac juice mixture by probiotic lactic acid bacteria. International Journal of Agricultural Technology, 14(7): 1455-1470.
Dimitrovski, Darko & Velickova, Elena & Langerholc, Tomaz & Winkelhausen, Eleonora. (2015). Apple juice as a medium for fermentation by the probiotic Lactobacillus plantarum PCS 26 strain. Annals of Microbiology.
Hossain, Mohammad & Hoque, M & Kabir, M & Yasin, Md. (2019). Probiotification of Mango Juice by Lactic Acid Bacteria and Quality Assessment at Refrigerated Storage Condition.
DLG (2017). Colours and their influences on sensory perception of products. http://www.evaderndorfer.at/pdf/DLG_3_2017_Expertenwissen_Sensorik_Farbe_englisch.pdf
Dhillon, H., Gill, M., Kocher, G., Panwar, H., & Arora, M. (2021). Preparation of Lactobacillus acidophilus enriched probiotic mango juice. Environmental Biology of Fishes. 42. 371-378.
Oomah, B.D. and L.S.Y. Liang, 2017. Volatile compounds of dry beans (Phaseolus vulgaris l.). Plant Foods Hum Ntur, 62: 177-183.
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